The car is expected to sell for more than $5 million, but its value is impossible to estimate because the car is so unique.
RM Auctions, the agency handling the sale of this car, sold a similar Bond car in 2005, but that car was used only for promotional purposes and not in the movies themselves.
One other 1964 DB5 was used in filming, but that car was stolen in 1997 and is believed to have been destroyed.
NEXT: Production history
Last updated June 01 2010: 2:55 PM ET